![]() (1992) Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis. ![]() (1991) Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Crop Protection 9, 351-4.īroglie, K., Chet, I., Holliday, M., Cressman, R., Biddle, P., Knowlton, S., Mauvais, J. (1990) Additive protective effects of different plant-derived insect resistance genes in transgenic tobacco plants. New York: Greene Publishing Associates/Wiley Interscience.īoulter, D., Edwards, G.A., Gatehouse, A.M.R., Gatehouse, J.A. (1989) Current Protocols in Molecular Biology. (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Plants expressing an insect chitinase gene may have agronomic potential for insect controlĪbel, P.P., Nelson, R.S., De, B., Hoffmann, N., Rogers, S.G., Fraley, R.T. However, both budworm and hornworm larvae, when fed on chitinase-expressing transgenic plants coated with sublethal concentrations of a Bacillus thuringiensis toxin, were significantly stunted relative to larvae fed on toxin-treated non-transgenic controls. In contrast, hornworm larvae showed no significant growth reduction when fed on the chitinase-expressing transgenics. Both parameters were significantly reduced when budworms fed on transgenic tobacco plants expressing high levels of the chitinase gene. Segregating progeny of high-expressing plants were compared for their ability to support growth of tobacco budworm (Heliothis virescens) larvae and for feeding damage. A truncated but enzymatically active chitinase was present in plants expressing the gene. ![]() ![]() This hypothesis was tested by introducing a cDNA encoding a tobacco hornworm (Manduca sexta) chitinase (EC 3.2.1.14) into tobacco via Agrobacterium tumefaciens-mediated transformation. Thus, insects feeding on plants that constitutively express an insect chitinase gene might be adversely affected, owing to an inappropriately timed exposure to chitinase. Chitinase expression in the insect gut normally occurs only during moulting, where the chitin of the peritrophic membrane is presumably degraded.
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